Functional and Structural Basis for a Bacteriophage Homolog of Human RAD52

M. Ploquin, A. Bransi, E. Paquet, A. Stasiak, A. Stasiak, X. Yu, A. Cieslinska, E. Egelman, S. Moineau; J.-Y. Masson

In eukaryotes, homologous recombination proteins such as RAD51 and RAD52 play crucial roles in DNA repair and genome stability. Human RAD52 is a member of a large single-strand annealing protein (SSAP) family and stimulates Rad51-dependent recombination. In prokaryotes and phages, it has been difficult to establish the presence of RAD52 homologs with conserved sequences. Putative SSAPs were recently found in several phages that infect strains of Lactococcus lactis. One of these SSAPs was identified as Sak andwas found in the virulent L. lactis phage ul36, which belongs to the Siphoviridae family. In this study, we show that Sak is homologous to the N terminus of human RAD52. Purified Sak binds single-stranded DNA (ssDNA) preferentially over double-stranded DNA (dsDNA) and promotes the renaturation of long complementary ssDNAs. Sak also binds RecA and stimulates homologous recombination reactions. Mutations shown to modulate RAD52 DNA binding affect Sak similarly. Remarkably, electron-microscopic reconstruction of Sak reveals an undecameric subunit ring, similar to the crystal structure of the N-terminal fragment of human RAD52. For the first time, we propose a viral homolog of RAD52 at the amino acid, phylogenic, functional, and structural levels.

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published January 7, 2009 in PLoS ONE